Romualdo Benigni, Selected Publications

→ Benigni, R. (2005) Structure-activity relationship studies of chemical mutagens and carcinogens: mechanistic investigations and prediction approaches. Chem.Revs., 105: 1767-1800.

→ Benigni, R., Bossa, C., Netzeva, T. I., and Worth, A. P. (2007) Collection and evaluation of (Q)SAR models for mutagenicity and carcinogenicity. EUR – Scientific and Technical Research Series; EUR 22772 EN. Luxenbourg, Office for the Official Publications of the European Communities; pp 1 – 119.

→ Benigni, R., Bossa, C., Jeliazkova, N., Netzeva, T., and Worth, A. P. (2008) The Benigni / Bossa rulebase for mutagenicity and carcinogenicity – a module of Toxtree. EUR – Scientific and Technical Research Series; EUR 23241 EN. Luxenbourg, Office for the Official Publications of the European Communities; pp 1 – 68.

→ Benigni R., Bossa C. (2008) Predictivity of QSAR. J.Chem.Inf.Model., 48: 971-980.

→ Benigni R., Bossa C. (2008) Structure Alerts for carcinogenicity, and the Salmonella assay system: a novel insight through the chemical relational databases technology. Mutat.Res.Revs., 659: 248-261.

→ Benigni, R., A. Worth, T. Netzeva, N. Jeliazkova, C. Bossa, A. Gruska, R. Franke (2009) Structural motifs modulating the carcinogenic risk of aromatic amines, Environ.Mol.Mutag., 50:152-161.

→ Benigni, R., Bossa, C., Tcheremenskaia, O,and Worth, A. P. (2009) Development of Structure Alerts for the in vivo micronucleus assay in rodents. Scientific and Technical Research Series; EUR 23844 EN. Luxenbourg, Office for the Official Publications of the European Communities; pp 1 – 42.

→ Benigni R., Bossa C., and Worth A. (2010) Structural analysis and predictive value of the rodent in vivo micronucleus assay results, Mutagenesis, 25: 335-341.

→ Benigni R., C. Bossa, O. Tcheremenskaia, and A. Giuliani (2010) Alternatives to the carcinogenicity bioassay: in silico methods, and the in vitro and in vivo mutagenicity assays. Expert Opinion on Drug Metabolism & Toxicology, 6: 1-11.

→ B., N. Douglas, C. Helma, M. Rautenberg, N. Jeliazkova, V. Jeliazkov, I. Nikolova, Benigni, R., O. Tcheremenskaia, S. Kramer, T. Girschick, F. Buchwald, J. Wicker, A. Karwath, M. Gütlein, A. Maunz, H. Sarimveis, G. Melagraki, A. Afantitis, P. Sopasakis, D. Gallagher, V. Poroikov, D. Filimonov, A. Zakharov, A. Lagunin, T. Gloriozova, S. Novikov, N. Skvortsova, D. Druzhilovsky , S. Chawla, I. Ghosh, S. Ray, H. Patel, S. Escher (2010) Collaborative Development of Predictive Toxicology Applications, J. Chemoinformatics, 2: 7.

→ R. Franke, A. Gruska, C. Bossa, and Benigni, R. (2010) QSARs of Aromatic Amines: identification of potent carcinogens, Mutat.Res., 691: 27-40.

→ Benigni R. and Bossa C. (2011) Mechanisms of chemical carcinogenicity and mutagenicity: a review with implications for predictive toxicology, Chem.Revs., 111: 2507-2536.

→ Benigni R. and Bossa C. (2011) Alternative strategies for carcinogenicity assessment: an efficient and simplified approach based on in vitro mutagenicity and cell transformation assays, Mutagenesis, 26: 455-460.

→ Benigni, R., Tcheremenskaia, O,and Worth, A. P. (2011) Computational Characterisation of Chemicals and Datasets in Terms of Organic Functional Groups – a new Toxtree Rulebase. Scientific and Technical Research Series; EUR 24871 EN. Luxenbourg, Office for the Official Publications of the European Communities; pp 1 – 34.


→ O. Tcheremenskaia, Benigni, R., I. Nikolova, N. Jeliazkova, S. E. Escher, M. Batke, T. Baier, V. Poroikov, A. Lagunin, M. Rautenberg and B. Hardy (2012) OpenTox Predictive Toxicology Framework: toxicological ontology and semantic media wiki-based OpenToxipedia, J.Biomed. Semantics, 3 Suppl. 1:S7.

→ O. G. Mekenyan, P. I. Petkov, S. V. Kotov, S. Stoeva, V. B. Kamenska, S. D. Dimitrov, M. Honma, M. Hayashi, Benigni, R., E. M. Donner, G. Patlewicz (2012) Investigating the relationship between in vitro – in vivo genotoxicity: Derivation of mechanistic QSAR models for in vivo liver genotoxicity and in vivo bone marrow micronucleus formation which encompass metabolism. Chem.Res.Toxicol., 25: 277 – 296.

→ Benigni R. (2012) Alternatives to the carcinogenicity bioassay for toxicity prediction: are we there yet?, Expert Opin.Drug Metab.Toxicol., 8: 1-11.

→ Benigni, R., C. Bossa, and O. Tcheremenskaia (2013) in vitro Cell Transformation assays for an integrated, alternative assessment of carcinogenicity: a data-based analysis, Mutagenesis, 28: 107-116.

Benigni R, Battistelli CL , Bossa C, Colafranceschi M, Tcheremenskaia O. (2013) Mutagenicity, Carcinogenicity, and Other End points. In Reisefeld B. and Mayeno A.N., Computational Toxicology: Vol. 2, Chapter 4; Methods Mol.Biol., 930: 67-98.

→ Benigni, R., C. Bossa, O. Tcheremenskaia (2013) Nongenotoxic Carcinogenicity of Chemicals: Mechanisms of Action, and Early Recognition through a new set of Structural Alerts. Chem.Rev., 113: 2940-2957.

→ M. Nendza, Silke Gabbert; Ralph Kühne; Anna Lombardo; Alessandra Roncaglioni; Emilio Benfenati; Romualdo Benigni; Cecilia Bossa; Sebastian Strempel; Martin Scheringer; Alberto Fernández; Robert Rallo; Francesc Giralt; Sabcho Dimitrov; Ovanes Mekenyan; Frank Bringezu; Gerrit Schüürmann (2013) A comparative survey of chemistry-driven in silico methods to identify hazardous substances under REACH. Regulatory Toxicol.Pharmacol., 66: 301- 314.

→ Benigni, R. (2013) Evaluation of the toxicity forecasting capability of EPA’s ToxCast Phase I data: can ToxCast in vitro assays predict carcinogenicity? J. Environ. Scie. Health, Part C, 31 (3): 201 – 212.

→ Benigni, R., Cecilia Bossa, Chiara Battistelli, Olga Tcheremenskaia (2013) IARC Class 1 and 2 carcinogens are successfully identified by an alternative strategy that detects DNA-reactivity and Cell Transformation ability of chemicals. Mutat.Res., 758: 56 – 61.

→ Benoît Schilter, Romualdo Benigni, Alan Boobis, Alessandro Chiodini, Andrew Cockburn, Mark Cronin, Elena Lo Piparo, Sandeep Modi, Anette Thiel, Andrew Worth (2014) Establishing the level of safety concern for chemicals in food without the need for toxicity testing. Regulatory Toxicol.Pharmacol., 68: 275 – 296.

→ Artem Cherkasov, Eugene N. Muratov, Denis Fourches, Alexandre Varnek, Igor I. Baskin, Mark Cronin, John Dearden, Paola Gramatica, Yvonne C. Martin, Roberto Todeschini, Viviana Consonni, Victor E. Kuz’min, Richard Cramer, Romualdo Benigni, Chihae Yang, James Rathman, Lothar Terfloth, Johann Gasteiger, Ann Richard, and Alexander Tropsha (2014) QSAR Modeling: Where have you been? Where are you going to?, J.Med.Chem., 57: 4977 – 5010.

→ Benigni, R. (2014) Predicting the carcinogenicity of chemicals with alternative approaches: recent advances. Expert Opin.Drug Metab.Toxicol., 10: 1199 – 1208.

→ Benigni R, Bossa C, Tcheremenskaia O, Battistelli CL, Giuliani A. (2015) The Syrian hamster embryo cells transformation assay identifies efficiently nongenotoxic carcinogens, and can contribute to alternative, integrated testing strategies. Mutat.Res., 779: 35-38.

→ Berggren E, Amcoff P, Benigni R, Blackburn K, Carney E, Cronin M, Deluyker H, Gautier F, Judson RS, Kass GE, Keller D, Knight D, Lilienblum W, Mahony C, Rusyn I, Schultz T, Schwarz M, Schüürmann G, White A, Burton J, Lostia AM, Munn S, Worth A. (2015) Chemical Safety Assessment Using Read-Across: Assessing the Use of Novel Testing Methods to Strengthen the Evidence Base for Decision MakingEnviron Health Perspect., 123: 1232 – 1240.

→ Benigni, R., C.L. Battistelli, C. Bossa, A. Giuliani, and O. Tcheremenskaia (2015) Alternative toxicity testing: analyses on Skin sensitization, Toxcast Phases I and II, and Carcinogenicity provide indications on how to model mechanisms linked to adverse outcome pathways. J. Environ. Scie. Health, Part C, 33: 422 – 433.

→ Benigni, R., C. Bossa, and O. Tcheremenskaia (2016) A data-based exploration of the adverse outcome pathway for skin sensitization points to the necessary requirements for its prediction with alternative methods. Regul.Toxicol.Pharmacol., 78: 45-52.

→ Benigni, R. (2016) Predictive toxicology today: the transition from biological knowledge to practicable models. Expert Opin.Drug Metab.Toxicol., 12: 989-992.

→ Benigni, R., C.L. Battistelli, C. Bossa, A. Giuliani, and O. Tcheremenskaia (2017) Endocrine disruptors: data-based survey of in vivo tests, predictive models, and the Adverse Outcome Pathway. Regul.Toxicol.Pharmacol., 86: 18-24.

→ R. Benigni, C. Bossa (2019) Data based review of QSARs for predicting genotoxicity: the state-of-the-art. Mutagenesis, 34: 17 – 23.

→ C. Hasselgren, E. Ahlberg, Y. Akahori, A. Amberg, L. T. Anger, F. Atienzar, S. Auerbach, L. Beilke, P. Bellion, R. Benigni, et al., (2019) Genetic toxicology in silico protocol. Regulatory Toxicology and Pharmacology, 107:104403.

→ R. Benigni (2019) Towards quantitative read across: Prediction of Ames mutagenicity in a large database. Regul. Toxicol. Pharmacol. 108:104434.

→ R. Benigni, R. Serafimova, J. M. Parra Morte, C. L. Battistelli, C. Bossa, A. Giuliani, E. Fioravanzo, A. Bassan, M. Fuart Gatnik, J. Rathman, C. Yang, A. Mostrag-Szlichtyng, O. Sacher, O. Tcheremenskaia (2020) Evaluation of the applicability of existing (Q)SAR models for predicting the genotoxicity of pesticides and similarity analysis related with genotoxicity of pesticides for facilitating of grouping and read across: an EFSA funded project. Regul. Toxicol. Pharmacol. 114:104658.